Editing N64 Toad's Turnpike

|image=[[File:Toad's Turnpike MK64.png|300px]]

The course takes place amid a very late sunset and is quite straightforward in terms of track layout. Players immediately leave a merging starting area to enter a figure-8-esque roadway featuring many moving vehicle obstacles; these include white [[Car (obstacle)|cars]], turquoise tanker [[Cargo truck|trucks]], red box trucks (which can carry three different cargo types), and yellow school [[Bus|buses]]. Players remain on this roadway for the rest of the race, never returning to the starting area. Each lap then consists of a 90° turn to the left, a U-turn to the left, a U-turn to the right, and a 90° turn to the right, with each turn being very long and gradual. At the beginning of the right U-turn, the roadway merges into two lanes, and remains this way until racers pass the finish line. Various points of the track have small openings with four [[Item Box]]es in each. A thin line of grass borders both sides of the track and is blocked off by a white fence. Should the player be knocked over the fence by items such as a [[Red Shell]], [[Lakitu]] will bring the player back to the main road. Beyond the tall walls that border the racetrack, billboards of Toad can be seen, making it his home track in the game. No other decorations can be found besides a lone Nintendo billboard and a large number of street lamps.

In 50cc, 100cc, and 150cc, the vehicles travel forward in the same direction as players. The engine class also affects how fast the vehicles move; traffic moves slowly in 50cc, but it is quite fast in 150cc. The difficulty resides in the fact that the larger vehicles go slowly through the road, sometimes blocking the player (especially where the roadway is two lanes, and vehicles often travel side-by-side, making it difficult to steer between them). In the Extra mode, the vehicles instead drive against players at the same speed as in 150cc. While each engine class in Versus races contains the same number of vehicles (seven of each kind, totaling 28), Time Trials feature one extra of each vehicle type on the course.

Item boxes, as well as coins, now move along the road, while the item boxes in the openings of the track are replaced with coins. The yellow school buses are now replaced by different colored transportation buses while the turquoise tanker trucks are gone. A few traffic helicopters are added, and the cars take a more realistic appearance, with some cars in different color schemes (not including the original white) based on Toyota Comfort taxis, popular in Japan, and the vehicles being scaled down to a size more proportionate to the racers. The empty [[Dash panel ramp truck|auto carrier trucks]] have Glide Ramps, and the pink pickup [[truck]]s have a surfboard which serves as a simple ramp. Vehicles in the left lane change lanes on every other lap of the course they complete, driving in the far left lane until the highway merges from three lanes into two; they indicate that they are changing lanes via orange turn-signal lights. Like in the original, the vehicles tumble over any racers that drive into them, but a [[Super Star]] launches them into the air and renders them harmless. Vehicles will also not tumble over any racers that are either driving too slowly or actively using a [[Boo]].

The third set has 33 vehicles. However, this set also reverses the direction of traffic in the right lane (left in Mirror Mode) so that it now travels against players.